The research focus of our group is to develop and apply quantitative proteomics methods in different biological areas. The aim of our study is the analysis of protein dynamics and post-translational modifications (PTMs) in cellular networks under regular and diseased conditions, such as type 2 diabetes mellitus and neurodegenerative disorders. We are also interested in transferring the SILAC technology to various model organisms, including worms, flies, zebrafish, and rodents.
Our group focusses on applying quantitative proteomics to analyse the dynamics of heart and skeletal muscle tissue. Skeletal muscles differ in their anatomical structure, neuronal innervation, and metabolic activity. Since muscle tissue make up approximately half of a person´s body weight its right function is essential for body shape, force production, and overall body homeostasis, including sugar uptake and energy production. In addition, skeletal muscle tissue is highly adaptable and is able to rapidly respond to environmental changes by modulating the synthesis and degradation of proteins.
Bock, T., Türk, C., Aravamudhan, S., Keufgens, L., Bloch, W., Rozsivalova, D.H., Romanello, V., Nogara, L., Blaauw, B., Trifunovic, A., Braun, T., Krüger, M. (2021). PERM1 interacts with the MICOS-MIB complex to connect the mitochondria and sarcolemma via ankyrin B. Nat Commun. 12(1), 4900.
Aravamudhan, S., Türk, C., Bock, T., Keufgens, L., Nolte, H., Lang, F., Krishnan, R.K., König, T., Hammerschmidt, P., Schindler, N., Brodesser, S., Rozsivalova, D.H., Rugarli, E., Trifunovic, A., Brüning, J., Langer, T., Braun, T., Krüger, M. (2021). Phosphoproteomics of the developing heart identifies PERM1 - An outer mitochondrial membrane protein. J Mol Cell Cardiol. 154, 41-59.
Tellkamp, F., Lang, F., Ibáñez Ricoma, A., Abraham, L., Quezada, G., Günther, S., Looso, M., Tann, F.J., Müller, D., Cemic, F., Hemberger, J., Steinfartz, S. and Krüger, M. (2020). Proteomics of Galápagos marine iguanas links function of femoral gland proteins to the immune system. Mol Cell Proteomics. 19(9), 1523-32.
Kallabis, S., Abraham, L., Müller, S., Dzialas, V., Türk, C., Wiederstein, J.L., Bock, T., Nolte, H., Nogara, L., Blaauw, B., Braun, T., and Krüger, M. (2020). High-throughput proteomics fiber typing (ProFiT) for comprehensive characterization of single skeletal muscle fibers. Skelet Muscle. 10, 7.
Lang, F., Khaghani, S., Türk, C., Wiederstein, J.L., Hölper, S., Piller, T., Nogara, L., Blaauw, B., Günther, S., Müller, S., Braun, T., Krüger, M. (2018). Single muscle fiber proteomics reveals distinct protein changes in slow and fast fibers during muscle atrophy. J Proteome Res. 17(10), 3333-47.
Nolte, H., MacVicar, T.D., Tellkamp, F., Krüger, M. (2018). Instant Clue: A Software Suite for Interactive Data Visualization and Analysis. Sci Rep. 8(1), 12648.
Wiederstein, J.L., Nolte, H., Günther, S., Piller, T., Baraldo, M., Kostin, S., Bloch, W., Schindler, N., Sandri, M., Blaauw, B., Braun, T., Hölper, S., Krüger, M. (2018). Skeletal Muscle-Specific Methyltransferase METTL21C Trimethylates p97 and Regulates Autophagy-Associated Protein Breakdown. Cell Reports 23(5), 1342-56.